Plate Reader

Amended to include set aside code. We require an instrument that can acquire real-time data from multiple modes of measurement, including monochromator-based fluorescence, UV/Vis absorbance, and luminescence. We require the following: linear Variable Monochromator, permitting tunable band passes up to 100nm; a tunable dichroic mirror permitting air-based light path for monochromator without light guides or fiberoptics; auto ranging of detector sensitivity and gain for fluorescence measurements below 760,000,000-counts; enhanced Dynamic Range autoranging of detector sensitivity in less than 0.1s; absorbance based on a dedicated spectrometer, not a monochromator. Complete UV/Vis absorbance spectrum from 220nm-1000nm at 1nm resolution in less than 0.5s with all wavelengths captured simultaneously with a diode array; heated optics. A heated optical head means that incubated microplate wells remain in a uniform temperature environment even during detection. The instrument should have a temperature controller than can go up to 45 C, bottom reading, two reagent injectors for automated injection for initiating kinetic events and flash luminescence, and an atmospheric control unit with software-controlled CO2 andO2 concentrations for cell culture at 5% CO2. The unit should come with software for data visualization and analysis.